librarian
librarian
- description
- Runs the
librarian
tool to derive the likely Illumina library preparation protocol used to generate a pair of FASTQ files. - help
- WARNING this tool is not guaranteed to work on all data, and may produce nonsensical results.
librarian
was trained on a limited set of GEO read data (Gene Expression Oriented). This means the input data should be Paired-End, of mouse or human origin, read length should be >50bp, and derived from a library prep kit that is in thelibrarian
database. This version oflibrarian
has been trained on "read one" data of Paired-End sequencing data. It is not intended for use with Single-End data, even though it only accepts a single FASTQ. - outputs
- {'report': 'A tar archive containing the
librarian
report and raw data.', 'raw_data': 'The raw data that can be processed by MultiQC.'}
Inputs
Required
_runtime
(Any, required)read_one_fastq
(File, required): Read one FASTQ of a Paired-End sample to analyze. May be uncompressed or gzipped.
Defaults
modify_disk_size_gb
(Int, default=0): Add to or subtract from dynamic disk space allocation. Default disk size is determined by the size of the inputs. Specified in GB.prefix
(String, default=sub(basename(read_one_fastq),"([_\.][rR][12])?(\.subsampled)?\.(fastq|fq)(\.gz)?$","") + ".librarian"): Name of the output tar archive. The extension.tar.gz
will be added.
Outputs
report
(File)raw_data
(File)